How can we avoid the precipitation in serum? -Huaqiang Electronic Network

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How can I avoid precipitation in serum?

Serum is the light yellow, transparent liquid that separates from plasma after blood coagulation, with fibrinogen removed. It plays a crucial role in cell culture by providing essential nutrients, hormones, growth factors, binding proteins, and protective components that help maintain cell health and prevent mechanical damage.

Main Functions of Serum:

• Provides Basic Nutrients: Amino acids, vitamins, inorganic substances, lipids, and nucleic acid derivatives are all necessary for cell growth and development.
• Delivers Hormones and Growth Factors: Insulin, dexamethasone, estradiol, testosterone, and other growth factors like FGF, EGF, and PDGF support cellular proliferation and differentiation.
• Offers Binding Proteins: Proteins such as albumin and transferrin carry important molecules like vitamins, hormones, and iron, supporting cellular metabolism and function.
• Protects Cells from Mechanical Damage: Serum provides a protective layer, especially during suspension cultures, reducing the risk of physical stress on cells.
• Contains Anti-Protease Components: Some cells release proteases, but serum contains inhibitors that neutralize them, helping to maintain cell integrity during processes like trypsin digestion.

How to Prevent Precipitation in Serum:

1. Avoid Heat Inactivation if Not Necessary: Heat inactivation can cause sedimentation, so skip this step unless absolutely required.
2. Follow Proper Heat Inactivation Protocol: If you must inactivate serum, do it at 56°C for 30 minutes, shaking it regularly to prevent uneven heating and sediment formation.
3. Thaw Serum Slowly and Mix Well: Once thawed, gently mix the serum to ensure uniform temperature and composition, reducing the chance of precipitation.
4. Store Serum Properly: Shake the serum occasionally during freezing to maintain even temperature and composition distribution.
5. Do Not Thaw Rapidly from -20°C to 37°C: Sudden temperature changes can cause protein denaturation and precipitation.
6. Avoid Leaving Serum at 37°C for Long Periods: Prolonged exposure can lead to cloudiness and degradation of sensitive components, affecting serum quality.

We hope these tips help you manage serum more effectively. Shanghai Jinma Experimental Equipment Co., Ltd. guarantees both quality and competitive pricing. Feel free to reach out for more information!