How can we avoid the precipitation in serum? -Huaqiang Electronic Network

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How can I avoid precipitation?

Serum is the light yellow, transparent liquid obtained after blood coagulation by removing fibrinogen from plasma or by removing the fibrous protein from plasma. Its primary function is to provide essential nutrients, hormones, growth factors, binding proteins, and protective elements that help protect cells from mechanical damage during culture.

Main Functions of Serum:

1. Provides basic nutrients such as amino acids, vitamins, inorganic substances, lipids, and nucleic acid derivatives, which are essential for cell growth and development.

2. Supplies hormones and various growth factors like insulin, dexamethasone, estradiol, testosterone, and growth factors such as FGF, EGF, and PDGF, which regulate cellular functions and promote proliferation.

3. Offers binding proteins that transport important low-molecular-weight substances. For example, albumin carries vitamins, fats, and hormones, while transferrin transports iron. These proteins play a key role in cellular metabolism and homeostasis.

4. Provides pro-contact and stretching factors that protect cells from physical stress, especially during suspension culture.

5. Offers some protection against proteases released by certain cell types, such as endothelial and bone marrow-like cells. The anti-protease components in serum help neutralize these enzymes, preventing unwanted cell damage. This was first observed when using serum to stop trypsin digestion during cell passage.

Effective Ways to Prevent Precipitation:

1. Heat inactivation of serum may increase sediment formation. If not necessary, this step can be omitted.

2. If heat inactivation is required, follow the 56°C for 30-minute rule and shake the serum frequently. Excessive temperature, prolonged time, or uneven shaking can lead to more sediment.

3. When thawing serum, make sure to mix it thoroughly to ensure even temperature and composition, which helps reduce the risk of precipitation.

4. During storage, regularly shake the serum (avoid creating bubbles) to maintain uniformity in temperature and composition.

5. Avoid rapid thawing from -20°C to 37°C. Sudden temperature changes can cause proteins to denature and precipitate.

6. Do not leave serum at 37°C for extended periods. Prolonged exposure can cause cloudiness and degrade unstable components, reducing serum quality.

I hope this information helps you better understand how to prevent precipitation and maintain the quality of your serum. Shanghai Jinma Experimental Equipment Co., Ltd. guarantees both quality and competitive pricing. Feel free to contact us for more details!